Enhancing wound healing in cattle through the application of vitreous humour fluid and platelet-rich plasma

The aim of this work: to examine the impact of applying vitreous humour fluid as well as platelet-rich plasma (PRP) on cattle's wound healing. Twelve dairy cows, randomly divided into three groups, constituted the subjects for this research. Those cows consumed standard quantities—grains; alfalfa; straw—with ad libitum clean water. We collected Vitreous humour fluid from slaughtered cows, storing it at 4°C. Through withdrawing blood from the same cows and separating PRP from plasma, we prepared Autologous PRP. Furthermore, we prepared a mixture of PRP with vitreous humour fluid which has been subsequently applied to wound site. At 7, 14, and 21 weeks, we performed biopsies on the cows for histopathological examination. On day 7, the histopathological examination revealed improved tissue reorganization and proliferation in wounds treated with PRP and vitreous humour fluid. Furthermore, in comparison with the control group--at this same time point--collagen organization has been notably superior. Moving onto day 14: a complete or partial union of wound margins manifested in the two groups treated with PRP and vitreous humour fluid; also, better granulation tissue formation stood out significantly—an indicator that healing progress had been robustly effective. By day 21; complete epithelialization—alongside dense collagen bundles filling the wound site—signified successful healing of wounds treated specifically with the use of vitreous humor fluid. The findings of the presented work indicate that the utilization of vitreous humour fluid and PRP could facilitate the process of wound healing in cattle. Improvements in tissue reorganization, proliferation, and collagen organization were all found. In the process of developing wound healing therapies for cattle, such findings offer useful insights that can be further developed.