Evaluation of the sensitivity and specificity of monoclonal antibodies for detecting the thioredoxin antigen in hepatocellular carcinoma patients

Duong Quang  Huy; Ho Truong  Giang; Nguyen Thi  Diem; Vo Thi Bich  Thuy

doi:10.55214/25768484.v9i1.4305

Authors

Duong Quang Huy Institute of Genome Research, Vietnam Academy of Science and Technology,18 Hoang Quoc Viet, Cau Giay, Hanoi 100000, Vietnam, and 103 Military Hospital, Vietnam Military Medical University, Hanoi 100000, Vietnam. https://orcid.org/0009-0003-4490-1839
Ho Truong Giang 103 Military Hospital, Vietnam Military Medical University, Hanoi 100000, Vietnam, and Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi 100000, Vietnam. https://orcid.org/0009-0000-0534-3764
Nguyen Thi Diem Institute of Genome Research, Vietnam Academy of Science and Technology,18 Hoang Quoc Viet, Cau Giay, Hanoi 100000, Vietnam. https://orcid.org/0009-0002-2923-8867
Vo Thi Bich Thuy Institute of Genome Research, Vietnam Academy of Science and Technology,18 Hoang Quoc Viet, Cau Giay, Hanoi 100000, Vietnam, and Graduate University of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi 100000, Vietnam. https://orcid.org/0000-0001-6746-9573

The objective of this study was to evaluate the sensitivity and specificity of the monoclonal antibody of thioredoxin (mAb-TXN) for detecting the thioredoxin (TXN) antigen in serum samples from patients with hepatocellular carcinoma (HCC). To achieve this, the mAb-TXN, generated from hybridoma cells, was utilized to optimize the conditions for an indirect ELISA immunoassay. The sensitivity, specificity, positive predictive value, and negative predictive value of the assay were evaluated on the basis of stored patient medical records. The results showed that indirect ELISA using mAb-TXN exhibited a sensitivity of 93.61%, a specificity of 96%, a positive predictive value of 97.77%, and a negative predictive value of 88%. In conclusion, mAb-TXN is a valuable tool for detecting TXN antigens in serum samples from HCC patients, offering high sensitivity and specificity for clinical application.

Section

How to Cite

Huy, D. Q. ., Giang, H. T. ., Diem, N. T. ., & Thuy, V. T. B. . (2025). Evaluation of the sensitivity and specificity of monoclonal antibodies for detecting the thioredoxin antigen in hepatocellular carcinoma patients. Edelweiss Applied Science and Technology, 9(1), 1010–1018. https://doi.org/10.55214/25768484.v9i1.4305